Fully compatible with high count

Sumaiya88

HTS relies upon on simple culturing protocols, microwell platforms and liquid handling automatons. HTS experimental design allows fast readout and automated data processing. HCA is suitable for obtaining a comprehensive understanding of tissue and cell mechanic, utilizing (single cell) omics and high-resolution imaging tools. Computational models are used to examine mechanobiological processes controlling organoid growth, patterning or for validating results from organoid studies. Measurements and features derived from organoid culture are structuring those models.

For drug screening in 2D cell cultures, 384 or 1,536 well plates are commonly used, which reduces the amount of reagent used per well/assay while allowing testing of a wider range of drugs or drug concentrations within one Spain phone number list single plate. This is why these plate formats are also preferred in organoid HTS. Owing to the small dimensions of the wells in these plates, manual handling can be cumbersome and slow. These plate formats are, therefore, more commonly used in combination with liquid handling robots.



Complex organoid protocols are not well suited for automatisation using liquid handling robots. In the past 5 years, several microplate-compatible organoid protocols have been developed, for example, for breast cancer (Sachs et al., 2018), kidney organoids (Czerniecki et al., 2018), pancreas (Hou et al., 2018) or colon organoids (Du et al., 2020). A different approach to make organoid culturing more practical for HTS is the engineering of alternatives to traditional cell culture dishes. An early example of such an alternative, developed by Gracz et al., is a polydimethylsiloxane (PDMS)/polystyrene-based microraft array.